Table 1. Properties of yeast strains used in this study.
| Yeast strain | Description | Cross-linking of mtATPase evidenced by CN-PAGE | Mitochondrial morphology observed by FM | Cristae formation observed by EM | Generation time (h) |
| YRD15 | Parental strain (MATα, his3, ura3, leu2, [rho+]) | No | Wild-type reticulum | normal | 6.98±0.25 |
| mtGFP | Expresses GFP1 targeted to the mitochondrial matrix | No | Wild-type reticulum | ND | ND |
| γ-25-HcRed | Expresses γ-25-HcRed2 | Dimers present in DM | Fragmented with appearance of balls and rings | reduced | 10.08±0.33 |
| γ-25-DsRed | Expresses γ-25-DsRed2 | Dimers, trimers and tetramers cross-linking (data not shown; see also [11], [12]) | Fragmented with appearance of balls and rings | reduced | 13.09±0.88 |
| γ-25-GFP | Expresses γ-25-GFP2 [14] | ND | Wild-type reticulum | ND | 7.26±0.45 |
| γ-25-mRFP | Expresses γ-25-mRFP2 | No | Wild-type reticulum | normal | 7.41±0.39 |
| γ-25-HcRed-mtHcRed | Expresses the γ-25-HcRed2 and ‘free’ HcRed1 targeted to the mitochondrial matrix | Reduced levels of cross-linking in presence of free HcRed | Predominantly wild-type reticulum | ND | ND |
1
expression from genomic location;
2
expression from vector. CN-PAGE, clear native polyacrylamide gel electrophoresis; FM, fluorescence microscopy; EM, electron microscopy; ND, not determined. Generation times are average of triplicate determinations +/− SD.