Table 1.

Liquid chromatography tandem mass spectrometry parameters for mecamylamine, nicotine and nicotine metabolites in urine

Compounds	Q1 Mass (amu)	Q3 Mass (amu)	Dwell Time (msec)	Declustering Potential (volts)	Entrance Potential (volts)	Collision Entrance Potential (volts)	Collision Energy (volts)	Cell Exit Potential (volts)	Retention Time (± SD) N= 30
Nicotine	163.2	132.2	150	35	5.5	12	21	4	2.59 (± 0.027)
		84.2	150	35	4.5	12	29	4
Nicotine-d4	167.2	136.1	150	35	6	12	21	4	2.56 (± 0.028)
		121.0	150	35	6	12	29	4
Cotinine	177.2	98.1	150	41	3	12	29	4	2.99 (± 0.030)
		80.1	150	41	3	12	33	4
Cotinine-d3	180.2	101.2	150	36	3	12	31	4	3.01 (± 0.029)
		80.2	150	36	3	12	33	4
OH-Cotininea	193.2	134.0	150	46	8	12	27	4	2.22 (± 0.021)
		80.2	150	46	8	12	35	4
OH-Cotinine-d3b	196.2	134.1	150	46	10.5	14	27	4	2.22 (± 0.021)
		79.9	150	46	10.5	14	38	4
Norcotinine	163.2	118.2	150	46	10.5	12	29	4	2.33 (± 0.023)
		80.2	150	41	10	12	33	4
Norcotinine-d4	167.2	139.2	150	46	10.5	12	29	4	2.34 (± 0.023)
		84.2	150	46	10.5	12	33	4
Mecamylamine	168.2	81.2	150	20	4	15	28	4	5.32 (± 0.016)
		137.2	150	20	4.5	15	16	4
Mecamylamine-d3	171.0	81.0	150	25	4	15	30	3	5.30 (± 0.017)
		137.2	150	25	4	15	16	3

^a: trans-3′-hydroxycotinine,

^b: trans-3′-hydroxycotinine-d₃

Underlined mass depicts quantification transition.