Table 5.
Mean ±SD stability of mecamylamine, nicotine and nicotine metabolites in urine under different specimen storage conditions
| Storage condition N = 5 |
% Analyte decrease | ||||
|---|---|---|---|---|---|
| Nicotine | Cotinine | OH-Cotininea | Norcotinine Mecamylamine | ||
| 24 h RTb | |||||
| Low | 4.0 ± 6.3 | 2.0 ± 10.2 | 9.2 ± 6.3 | 11.5 ± 3.7 | 1.1 ± 9.4 |
| Medium | 12.3 ± 8.1 | 0.8 ± 6.4 | 0.6 ± 8.8 | 8.9 ± 8.6 | 0.5 ± 1.9 |
| High
|
4.5 ± 3.3 | 3.3 ± 7.7 | 0.5 ± 3.2 | 7.7 ± 5.2 | 0.8 ± 2.8 |
| 72 h 4°C | |||||
| Low | 3.8 ± 7.8 | 14.3 ± 23.5 | 2.2 ± 12.2 | 3.5 ± 4.3 | 2.9 ± 21.1 |
| Medium | 0.9 ± 7.1 | 0.6 ± 5.4 | 1.9 ± 4.4 | 6.4 ± 5.1 | 5.5 ± 14.4 |
| High
|
4.6 ± 6.4 | 1.0 ± 4.9 | 2.0 ± 3.5 | 2.1 ± 3.1 | 0.1 ± 2.3 |
| 72 h 15°C Autosampler | |||||
| Low | 0.4 ± 5.9 | 7.2 ± 12.3 | 0.6 ± 4.8 | 1.4 ± 8.7 | 3.0 ± 8.2 |
| Medium | 1.5 ± 1.8 | 1.4 ± 4.1 | 2.8 ± 7.7 | 0.5 ± 4.5 | 1.3 ± 2.8 |
| High
|
3.4 ± 5.9 | 0.6 ± 4.8 | 0.7 ± 5.4 | 1.7 ± 4.4 | 0.1 ± 2.7 |
| 3 Freeze thaw cycles | |||||
| Low | 10.8 ± 9.6 | 4.0 ± 11.2 | 5.8 ± 19.7 | 2.5 ± 3.3 | 7.0 ± 13.1 |
| Medium | 7.3 ± 5.3 | 1.6 ± 10.3 | 7.4 ± 1.4 | 0.1 ± 5.2 | 4.0 ± 3.8 |
| High | 8.6 ± 4.3 | 3.5 ± 3.7 | 7.1 ± 6.4 | 1.9 ± 2.4 | 0.2 ± 1.4 |
a
trans-3′-hydroxycotinine,
b
room temperature