Skip to main content
. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Genes Brain Behav. 2012 Feb 15;11(3):332–341. doi: 10.1111/j.1601-183X.2012.00768.x

Figure 4.

Figure 4

(a) Patients with FXS (n=4) display increased levels of phosphorylated Threonine 389 (T389) p70 S6 kinase 1 (S6K1) compared to normal controls (n=4, p=0.0274). The percent (%) phospho-signal normalized to total protein signal for each graph. (b) Representative western blot images for pT389 P70 S6K, P70 S6K, GAPDH (loading control) from lysates from four patient sets. (c) Patients with FXS show increased pS473 Akt (PKB) levels in the brain compared to normal controls (p=0.0298). The percent (%) of phospho-signal was normalized to total protein signal for graph. (d) Representative western blot images for pS473 AKT and total AKT from lysates from four patient sets. (FXS, n=4; N, n=4) (e) Patients with FXS (n=4) show no difference in pERK1/2 levels compared to normal controls (n=4), for both pERK1 or pERK2. The percent (%) of phospho-signal normalized to total protein signal for graph. (f) Representative western blot images for pERK 1/2 total ERK1/2 from lysates from four patient sets. (g) Levels of CYFIP2 in the frontal lobe are not different than normal controls. CYFIP2 (145 kD band) normalized to total GAPDH protein signal for graph (FXS, n=4; normal, n=4). (h) Representative western blot images for CYFIP2, GAPDH (loading control) from lysates from four patient sets. The presence of the larger band ~150 kD was only seen in brain derived samples. All ECL signal detection was non-saturation (65K bit detection, GE Las400 imager). The error bars represent standard error in each graph. Blots were checked for efficient stripping prior to re-probing.