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. Author manuscript; available in PMC: 2013 Apr 3.
Published in final edited form as: Anal Chem. 2012 Mar 6;84(7):3040–3048. doi: 10.1021/ac3000573

Figure 6.

Figure 6

ESI ECD tandem mass spectrum of an O-glycosylated peptide from immunoglobulin type A1 hinge region peptide55. The spectrum shows that 11 of the 30 peptide bonds are cleaved. The sites of mucin-type O-glycosylation on Ser/Thr residues as determined from the tandem mass spectrum are shown. Open squares = GalNAc, shaded circles = Gal. The m/z values of the product ions identified four glycosylation positions unequivocally. One GalNA modifies either of the two positions indicated; the data were not sufficient to differentiate the two possibilities. ©2005, American Society for Biochemistry and Molecular Biology, used with permission.