| PVP (polyvinylpyrrolidine) | 50 mg |
| L-tyrosine | 0.5 mg |
| Stock salts | 840 μl |
| .25M Hepes pH 7.4 | 1000 μl |
| Inulin (5 mg/ml in H2O) | 1000 μl |
| Ammo acid stock | 1250 μl |
| Tissue-culture grade H2O | 1320 μl |
| Fetal bovine serum (FBS) | 4000 μl |
| LPSR-1 (Sigma L9263) | 1000 μl |
| (optional: reduce FBS to 3 ml if LPSR-1 is used) | |
| Penicillin-streptomycin (Sigma P3539) | 100 μl |
| Galactose stock | 100 μl |
| Base mix | 100 μl |
| L-glutamine (14 mg/ml in H2O; mix fresh) | 100 μl |
| BME vitamins (Sigma B6891) | 50 μl |
| 0.5M Na2 HPO4 | 40 μl |
| Pyruvic acid (14 mg/ml in H2 O; mix fresh) | 20 μl |
| 1 M MgSO4 | 20 μl |
| 1 M CaCl2 (optional) | 20 μl |
| Lactate syrup (Sigma L4263) | 10 μl |
| Trace minerals | 10 μl |
| Chicken egg yolk dilution or cholesterol 3-sulfate stock | 50 μl |
| or chemically defined lipid mix, Gibco BRL or Sigma L-5146 | 100 μl |
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