Figure 5.

Targeted path scanning in Yellow Cameleon 3.6 cultures confirms variable IIS trajectories. A, Raster plot of calcium traces (corresponding to the burst shown in B) illustrates variable activation times for individual cells. B, C, Panels depict the unique activation order of cells (colored circles) during two of the bursts represented in the trajectories. Cell color encodes the time of initial spike-related increase in calcium relative to the peak of the population spike in the field recording (0 ms). D, Neurons activate in unique sequences during subsequent IISs. Calcium imaging data from the events marked with asterisks in the electrical field record (top) were plotted as trajectories by calculating the spatial average of all activating cells at each time step (26 ms).