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. Author manuscript; available in PMC: 2012 Apr 4.
Published in final edited form as: Mol Cell. 2011 Oct 21;44(2):265–278. doi: 10.1016/j.molcel.2011.07.037

Figure 2. Granzyme B-dependent proteolysis of IL-1α enhances cytokine bioactivity.

Figure 2

(A) HeLa (top panels) or HUVEC cells (lower panels) were incubated with 1 nM of recombinant IL-1α104-271 or Annexin V as a control protein. Culture supernatants were collected at the indicated time-points, with IL-6, IL-8 and GM-CSF concentrations then determined by ELISA.

(B) HeLa or HUVEC cells were incubated with the indicated concentrations of full-length or granzyme B-cleaved IL-1α for 8h and concentrations of IL-6, IL-8 and GM-CSF in the supernatants were determined by ELISA.

(C) HeLa cells were left untreated or incubated with 2 nM of full-length or granzyme B-cleaved IL-1α. Culture supernatants were collected at the indicated time-points, with IL-6 and IL-8 concentrations subsequently determined by ELISA. All results are representative of at least three independent experiments. Error bars represent the mean ± the SEM of triplicate experiments.