Skip to main content
. Author manuscript; available in PMC: 2012 Apr 4.
Published in final edited form as: J Pharm Sci. 2009 Oct;98(10):3562–3574. doi: 10.1002/jps.21686

Figure 4.

Figure 4

Figure 4

Figure 4

CD spectra of EC1 in the absence and presence of DTT after incubation at 4 °C for 0, 14, or 28 days at pH (A) 3.0, (B) 7.0, and (C) 9.0. (A) In the absence of DTT at pH 3.0, EC1 undergoes secondary structural change presumably due to dimerization and oligomerization. In the presence of DTT, there is no secondary structural change in EC1. (B) At pH 7.0 on day 28, there is some loss of the β-sheet structure of EC1 in the absence of DTT as seen from the decrease in the minimum at 216 nm; addition of DTT suppresses the secondary structural change of EC1. (C) At pH 9.0 on day 28, the CD spectra remain unchanged in the presence and absence of DTT.