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. Author manuscript; available in PMC: 2012 Apr 4.
Published in final edited form as: J Pharm Sci. 2009 Oct;98(10):3562–3574. doi: 10.1002/jps.21686

Figure 5.

Figure 5

Figure 5

Figure 5

Thermal unfolding profiles of EC1 in the absence and presence of DTT measured by CD at 218 nm after incubation for 14 to 28 days at 4 °C at (A) pH 3.0, (B) 7.0, and (C) 9.0. (A) At pH 3.0, the thermal unfolding profiles in the absence of DTT are different on day 14 than on day 0. In the presence of DTT, the profiles look the same on days 0 and 14. (B) At pH 7.0, the thermal unfolding profiles in the absence of DTT are different at different time points; however, the profiles are relatively the same in the presence of DTT. (C) At pH 9.0, the thermal unfolding profiles are comparable after incubation for 14 and 28 days in the presence or absence of DTT.