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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Exp Cell Res. 2012 Mar 3;318(8):867–876. doi: 10.1016/j.yexcr.2012.02.022

Figure 1. p53 binds to the rOC promoter region.

Figure 1

(A) The rat OC promoter region contains a putative p53 response element at the position of −690 to −667 bp. The TATA box site is also indicated. Black boxes and Numbers, exons. (B) EMSA assay. NE, nuclear extracts from p53 over-expressed ROS17/2.8 cells; Labeled, Biotin-labeled probes; Unlabeled, competitive probes. (C) ChIP assay. Rat ROS17/2.8 cells were cross-linked, sonicated and then immunoprecipitated using an anti-p53 monclonal antibody. The known p53RE sequence in the rat p21 promoter was used as a p53RE positive control. Input, sonicated and precleared supernatant; IP-p53, p53 antiboby-immunoprecipitated samples; IgG, negative controls treated with normal mouse IgG antibody.

Suggestion for this figure: use a TRANSFAC matrix motif in place of the old paper, as it is more current and gives a better representation of the possible nucleotides at each location. Also, for 1B, you cannot be certain that p53 is binding to the EMSA motif as there is no supershift by an antibody specific to p53 and a cell lysate was used.