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. Author manuscript; available in PMC: 2012 Apr 4.
Published in final edited form as: J Immunol. 2011 Aug 12;187(6):3267–3276. doi: 10.4049/jimmunol.1002809

Figure 5. The P2 anchor residue affects cytolytic activity by Ly49Wi2-expressing RNK-16 cells.

Figure 5

A. RMA-S cells stably transfected with RT1-A1c (RMA-S.A1c) were cultured overnight at 26°C and then incubated in the presence of 10 μM P2P, P2Q, P2V or no peptide at 37°C for 4 hrs. Cells were then stained with YR5/12 (black histograms) or isotype control (grey histograms) followed by secondary staining with FITC conjugated anti-rat IgG. Cells were analyzed by flow cytometry. The MFI are indicated on the top right corner of each histogram. B. RNK-16 cells were stably transfected with the chimeric recptor Ly49Wi2 (RNK.49Wi2) which retains the recognition domain of Ly49i2, but possesses the activation domain of Ly49W. Expression was confirmed by staining cells with STOK2. C. RNK.49Wi2 cells were used as effector cells in a 4-hr cytotoxicity assay with RMA-S.A1c target cells incubated with P2P, P2Q, P2V, or no peptide.