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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: J Cell Biochem. 2012 Apr;113(4):1245–1252. doi: 10.1002/jcb.23455

Figure 2. sGAG production by MSC pellet cultures during chondrogenesis.

Figure 2

Pellet cultures of MSCs, transfected with full length COMP cDNA (COMP) or control vector (Control), were induced to undergo chondrogenic differentiation, harvested at the indicated time points, and analyzed for sGAG content using the Blyscan kit. DNA contents of the pellets were determined using PicoGreen assay and used for normalization of sGAG contents. The results showed that COMP over-expression resulted in higher sGAG production during the first 2 weeks of MSC chondrogenesis. Values are mean ± SD (n = 5).