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(A) VICE was applied, and levels of Nanog mRNA were measured by real-time RT-PCR and normalized to levels of β-actin mRNA (top). Representative image of the RT-PCR end-product (bottom). (B) The frequency of Nanog+ cells at each stage of immunoediting was measured by immunofluorescence analysis. (C) The percentage of Nanog+ cells was quantified. Data are representative of three independent experiments.
