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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Cancer Res. 2012 Feb 14;72(7):1717–1727. doi: 10.1158/0008-5472.CAN-11-3758

Figure 4. Contribution of Nanog to the growth and stem-like properties of immune-resistant cancer cells after vaccination.

Figure 4

(A) live P3 cells treated with siGFP or siNanog were counted at each time point. (B) Cell cycle stages of P3 cells was measured by flow cytometry (left). The expression of various cell cycle proteins was determined (right). (C) The treated P3 cells were cultured in low density suspension conditions to promote sphere formation (left). The number of spheres per well was quantified (right). (D) The live P0 cells were counted at each time point after trypan blue staining. (E) The frequency of the transduced P0 at different cell cycle stages was measured by flow cytometry (left). The expression of various cell cycle proteins was determined (right). (F) The transduced P0 were cultured in low density suspension conditions to promote sphere formation (left). The number of spheres per well was quantified (right). Data are representative of three independent experiments.