Figure 1. Flow cytometric identification of Tregs in papilloma TILs and peripheral blood.

PBMC and papilloma tissue were stained and analyzed for surface expression of CD4, CD25, CD127, and intracellular Foxp3. A representative staining of PBMC (top row) and TILs (bottom row) from a patient with RRP is shown. A) The ungated events (all cells) from these tissues; B) forward and side scatter characteristics of CD4⁺ gated cells; C–E) CD25, CD127, and Foxp3 expression of CD4⁺ lymphocytes. A gate is drawn around CD4⁺ cells which are CD25⁺CD127^low/− (C) and the Foxp3⁺CD127^low/− (D); E) The correlation of CD25⁺ and Foxp3⁺ on CD4⁺ T-cells. The percentages of CD4⁺ cells are indicated for each subset.