A. nucleotide (underlined) and amino acid sequence of Ainp1. B. Ainp1 mRNA is expressed in most human tissues. Northern blot analysis using Ambion FirstChoice human pre-made blot was performed as previously described [19]. 1, brain; 2, placenta; 3, skeletal muscle; 4, heart; 5, kidney; 6, pancreas; 7, liver; 8, lung; 9, spleen; 10, colon. A 500 bp antisense cDNA fragment of Ainp1 was used. β-actin was used as the standard for comparison.