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. Author manuscript; available in PMC: 2013 Jul 1.

Published in final edited form as: Cancer Lett. 2012 Feb 2;320(1):111–121. doi: 10.1016/j.canlet.2012.01.037

Fig. 6 — Ainp1 caused ARNT-dependent Hep3B cell death. Cells were transfected with either GFP or GFP-Ainp1 expressing plasmid, followed by treatment with water (A) or 100 μM CoCl₂ (B). C, same as A except that pSport or pSport-ARNT plasmid was included in the transfection mix. Total cell count was determined by CellTiter assay at various time points (0-60 h). At zero time point, all conditions were started at a similar total cell count in all cases (A-C). The total amount of plasmid DNA (0.2 μg) in all conditions was the same in A-C so that a lesser amount of GFP or GFP-Ainp1 plasmid (0.1 μg) was used to accommodate pSport or pSport-ARNT plasmid (0.1 μg). Absorbance at 560 nm (y-axis) corresponds to the total cell number. This experiment was repeated once with similar results. Error bars indicate the variations of the means (mean ± SD, n = 3). **p < 0.01, ****p < 0.0001.

Fig. 6 — Ainp1 caused ARNT-dependent Hep3B cell death. Cells were transfected with either GFP or GFP-Ainp1 expressing plasmid, followed by treatment with water (A) or 100 μM CoCl₂ (B). C, same as A except that pSport or pSport-ARNT plasmid was included in the transfection mix. Total cell count was determined by CellTiter assay at various time points (0-60 h). At zero time point, all conditions were started at a similar total cell count in all cases (A-C). The total amount of plasmid DNA (0.2 μg) in all conditions was the same in A-C so that a lesser amount of GFP or GFP-Ainp1 plasmid (0.1 μg) was used to accommodate pSport or pSport-ARNT plasmid (0.1 μg). Absorbance at 560 nm (y-axis) corresponds to the total cell number. This experiment was repeated once with similar results. Error bars indicate the variations of the means (mean ± SD, n = 3). **p < 0.01, ****p < 0.0001.