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. Author manuscript; available in PMC: 2013 Jun 15.
Published in final edited form as: N Biotechnol. 2011 Dec 6;29(5):526–533. doi: 10.1016/j.nbt.2011.11.015

FIGURE 4.

FIGURE 4

Probing of SH3 domain array with the G9 monobody. Four membranes (purchased from Panomics) were incubated with His6-tagged SUMO fusion of the G9 monobody, washed, incubated with anti-His6-tag antibody conjugated to HRP, followed by detection with Enhanced Chemiluminescence-Plus (ECL-Plus). Panels A-D correspond to Panomics Arrays I-IV, respectively. Of the 150 SH3 domains tested, only the Fyn SH3 domain (duplicate, adjacent spots in panel A) bound to the G9 monobody. A His6-tagged ligand has been spotted in duplicate along the right side and the bottom of each membrane; these positive control spots are intended for alignment.