Table 1. Primers used in this work.

Primer	Sequence (5′→3′)	RS*
AHM	CAATTTCACACAGGAAACAGC	-
FM	TGTAAAACGACGGCCAGT	-
1025-HindIII	cccaagcttgggCGCTTCCGTTCCTCAATAG	HindIII
1025-XbaI	gctctagagcGCTCGACGACCCGCTTTG	XbaI
2759-HindIII	cccaagcttgggCCCAATATCGAGGTCACGT	HindIII
2759-XbaI	gctctagagcCGCCGCACCGATCAATAAG	XbaI
1025-NcoI	catgccatggtcATGTTTACTTCCGGACGAGAATATAAAG	NcoI
1025-XhoI	ccgctcgagcggTTACGGGGAGACGCCGGTAAC	XhoI
2759-5	atcgggtctcccATGTCATTTGCTTCCACGCTAAAAAAAG	BsaI
2759-3	atcgggtctcgaattTTATTTACGTTGGTTAAGCATC	BsaI

Underlined, the added oligosequence flanking the hybridization site.

*RS, restriction site (in bold).