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Transduction of dendritic cells with lentiviral vectors expressing green fluorescent protein was evaluated by flow cytometry. Dendritic cells (DCs) were seeded in six-well plates at 1 × 106 cells/well. Lentiviral vectors ubiquitinated hepatitis B virus core antigen (LV-Ub-HBcAg), lentiviral vectors hepatitis B virus core antigen (LV-HBcAg) or lentiviral vector (LV) was added at an multiplicity of infection of 20. GFP: Green fluorescent protein.
