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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Nat Immunol. 2011 Nov;12(11):1045–1054. doi: 10.1031/ni.2131

Figure 3. Lin CD127+ CD25+ ST2+ ILCs in human lung and airways.

Figure 3

(a,c) Flow cytometric gating strategy for identifying CD127+ Lin ILCs (CD3 TCRαβ CD11c CD11b CD19 CD56) in human lung parenchyma tissue (lower lobe) (a) or bronchoalveolar lavage (BAL) fluid (c), plots gated on live cells. (b,d) Expression of CD25 and ST2 on lineage negative CD127+ human lung parenchyma cells (b) and BAL cells (d) (black line) compared to FMO controls (gray shaded). FMO = fluorescence minus one. For examination of human BAL, data shown is representative of 7 of 9 lung transplant recipient patients examined. Data from lung parenchyma is representative of four cadaver tissue donors.