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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Nat Immunol. 2011 Nov;12(11):1045–1054. doi: 10.1031/ni.2131

Figure 3. Lin− CD127+ CD25+ ST2+ ILCs in human lung and airways.

Figure 3

(a,c) Flow cytometric gating strategy for identifying CD127+ Lin− ILCs (CD3− TCRαβ− CD11c− CD11b− CD19− CD56−) in human lung parenchyma tissue (lower lobe) (a) or bronchoalveolar lavage (BAL) fluid (c), plots gated on live cells. (b,d) Expression of CD25 and ST2 on lineage negative CD127+ human lung parenchyma cells (b) and BAL cells (d) (black line) compared to FMO controls (gray shaded). FMO = fluorescence minus one. For examination of human BAL, data shown is representative of 7 of 9 lung transplant recipient patients examined. Data from lung parenchyma is representative of four cadaver tissue donors.