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. Author manuscript; available in PMC: 2013 Mar 20.
Published in final edited form as: Anal Chem. 2012 Mar 2;84(6):2883–2891. doi: 10.1021/ac203434x

Figure 2.

Figure 2

Design of a vertical-flow device for detecting and quantifying i) the levels of total serum protein (Protein), ii) alkaline phosphatase (ALP), and iii) aspartate aminotransferase (AST) in blood obtained from a fingerstick. (a) An oblique view of the assembled device. The device comprises three inexpensive, lightweight, and readily available components: Fellows® single-sided, self-adhesive cold lamination sheets (the top and bottom sheets used to encase the paper holding the reaction zones), Whatman Grade 1 Chromatography paper (patterned to create three reaction zones), and a Pall plasma separation membrane (PSM) (Vivid GX PSM for filtering red blood cells). b) Paper patterned with three circular hydrophilic zones (d ~ 2 mm) surrounded by a hydrophobic wax barrier.