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. 2011 Nov 18;18(1):231–243. doi: 10.2119/molmed.2011.00210

Figure 3.

Figure 3

MAPK and Akt kinase activities in control (C) lung explants and treated with ANGII at 10−9 mol/L (Ang 10−9), ZD-7155 (ZD) or PD-123319 (PD). (A) Western blot analysis of p38, p44/42, JNK1/2 and Akt and to diphosphorylated forms of p38 (dp-p38), p44/42 (dp-p44/42), SAPK/JNK (dp-JNK1/2) and Akt (dp-Akt). Control loading was performed using β-tubulin (55 kDa). p38 corresponds to 38 kDa. p44/42 correspond to 44 and 42 kDa, respectively. JNK1 and 2 correspond to 46 and 54 kDa, respectively. Akt corresponds to 56 kDa. Semiquantitative analysis for dp-p38 (B), dp-p44/42 (C), dp-JNK1/2 (D), and dp-Akt (E). Results are presented as arbitrary units normalized for β-tubulin. P < 0.05: *versus ANGII at 0 mol/L (control, C), §versus ANGII at 10−9 mol/L, †versus ZD-7155 at 10−5 mol/L.