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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Mol Cell Biochem. 2012 Jan 21;364(1-2):59–70. doi: 10.1007/s11010-011-1205-7

Fig 4.

Fig 4

Phosphorylation of eIF-2α. ARVMs were treated with ISO for 15 min, and ISO or THAP for 3 or 24 h. Cell lysates were analyzed by western blot using anti-phospho-specific eIF-2α antibodies. A. β-AR stimulation for 15 min increased eIF-2α phosphorylation. *p<0.05 vs. CTL; n=4. B. β-AR stimulation for 3 h decreased eIF-2α phosphorylation. THAP treatment (3 h) had no eIF-2α phosphorylation. *p<0.05 vs. CTL; n=3–5. C. Phosphorylation of eIF-2α remained lower 24 h after β-AR stimulation. THAP treatment (24 h) increased eIF-2α phosphorylation. *p<0.05 vs. CTL; n=5–6. The lower panels exhibit the mean data normalized to GAPDH.