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. 2012 Jan 30;158(4):1503–1513. doi: 10.1104/pp.111.192856

Figure 2.

Figure 2.

Activity tests for VirD2 and RecA proteins. A, The cleavage activity of the recombinant VirD2 protein was tested using a 5′ fluorescently labeled 4.8-kb ssT-DNA substrate in the presence or absence of 1 μg of VirD2. The efficiency of the cleavage reaction was calculated as the ratio of fluorescence of the cleavage product (16mer) to the total amount of fluorescence in the reaction (4.8-kb DNA + 16 mer). B, The single-stranded DNA binding activity of the RecA protein was tested using ΦX174 ssDNA as a substrate and various amounts of the RecA protein. The binding of the RecA protein to the ssDNA substrate resulted in ssDNA-RecA complexes, and retardation of the DNA mobility due to the presence of protein was proportional to the amount of protein bound to DNA.