Figure 6.

Southern-blot analysis of the transgene copy number and integration patterns in transgenic triticale plants that were regenerated from microspores transfected with the nano-complex (VirD2-ssT-DNA-RecA + Tat₂). gDNA was digested with BamHI and hybridized with the GUS-specific probe. Lane Ult wt, Nontransgenic triticale plant var Ultima (wild type); lane C, positive control (the 4.8-kb GUS transgene cassette); lane M, DIG-labeled DNA size markers. The numbers above other lanes indicate the regenerated plant lines. Asterisk, Head-to-tail or tail-to-head integration event (4.8 kb); ^, Head-to-head or tail-to-tail integration event (4.6 kb).