Table I. Plant regeneration.
Plant regeneration from embryos obtained from control (untreated) microspores and from microspores treated with various DNAs and DNA/protein complexes in the presence or absence of the Tat2 peptide. Numbers in the table represent average values (± sd) from four (treatments with Tat2) or three (treatments without Tat2) independent experiments.
| Treatment | Embryos Plated | Abortive Embryos | Rooted Embryos | Albino Plantlets | Green Plantlets | Regeneration Efficiency |
| % | ||||||
| No treatment | 90.8 ± 7.4 | 49.3 ± 14.2 | 31.0 ± 14.4 | 4.3 ± 3.0 | 6.0 ± 2.8 | 6.6 ± 3.0 |
| No DNA + Tat2 | 113.3 ± 24.8 | 73.8 ± 19.7 | 27.5 ± 16.3 | 5.3 ± 2.6 | 6.8 ± 6.1 | 6.1 ± 5.7 |
| pACT-1D/PstI + Tat2 | 140.8 ± 39.5 | 71.3 ± 17.9 | 45.5 ± 21.0 | 10.3 ± 5.6 | 16.3 ± 14.2 | 11.5 ± 10.5 |
| dsT-DNA + Tat2 | 163.0 ± 3.5 | 88.8 ± 19.3 | 46.3 ± 11.8 | 12.5 ± 4.4 | 9.5 ± 1.3 | 5.9 ± 0.9 |
| ssT-DNA + Tat2 | 85.5 ± 36 6 | 35.3 ± 26.4 | 37.3 ± 4.8 | 7.5 ± 1.9 | 7.8 ± 7.6 | 8.3 ± 6.2 |
| ssT-DNA-RecA + Tat2 | 126.8 ± 35.3 | 73.8 ± 37.8 | 31.8 ± 2.6 | 12.5 ± 4.5 | 6.3 ± 1.0 | 5.4 ± 1.9 |
| VirD2-T-DNA + Tat2 | 126.3 ± 42.6 | 82.0 ± 36.2 | 33.0 ± 6.4 | 5.0 ± 6.0 | 6.3 ± 3.0 | 4.9 ± 1.4 |
| VirD2-T-DNA-RecA + Tat2 | 173.0 ± 73.1 | 113.0 ± 40.7 | 33.0 ± 20.7 | 21.5 ± 16.7 | 5.3 ± 3.6 | 3.0 ± 1.5 |
| No DNA | 107.7 ± 42.4 | 74.3 ± 34.0 | 18.7 ± 17.7 | 9.7 ± 15.9 | 5.0 ± 6.1 | 6.6 ± 9.4 |
| pACT-1D/PstI | 109.7 ± 34.5 | 79.7 ± 14.0 | 22.0 ± 24.3 | 5.0 ± 4.6 | 3.0 ± 2.6 | 2.5 ± 2.2 |
| dsT-DNA | 74.3 ± 6.7 | 59.7 ± 11.0 | 5.3 ± 2.1 | 8.0 ± 6.2 | 1.3 ± 2.3 | 1.8 ± 3.1 |
| VirD2-ssT-DNA-RecA | 70.7 ± 20.2 | 50.0 ± 6.6 | 12.7 ± 11.0 | 4.7 ± 7.1 | 3.3 ± 5.8 | 3.5 ± 6.1 |