Table III. Comparison of the transgene copy number and transgene expression.

The transgene copy number and transgene expression were compared in plants that were regenerated from microspores transfected with various types of T-DNA and T-DNA/protein complexes in the presence of the Tat₂ CPP.

Treatment/Plant Line	Protein Amounta	BamHI Fragment Sizes				Transgene Copy No.	Integration Locus No.
		kb
dsT-DNA
23	50.3 ± 2.5	5.0	6.4	7.8		3	3
61	29.0 ± 3.2	2.8	∼20			2	2
63	50.7 ± 3.5	4.8b	7.4	14.4		4	3
152	30.6 ± 1.8	4.4	4.6c	10.8		4	3
190	43.5 ± 2.2	4.8b	5.4	5.6	7.6	5	4
						3.6 ± 1.1	3.0 ± 0.7
VirD2-ssT-DNA-RecA
225	12.3 ± 1.2	8.8				1	1
237	45.7 ± 3.4	4.8b	19.2			3	2
241	43.5 ± 2.2	4.4	4.6c	7.4	12.0	5	4
267	2.5 ± 0.5	5.8				1	1
269	36.6 ± 2.1	2.6	15.0			2	2
						2.4 ± 1.7	2.0 ± 1.2
ssT-DNA
55	28.3 ± 2.6	5.3	9.4			2	2
ssT-DNA-RecA
51	11.1 ± 0.8	11.0				1	1
88	0.8 ± 0.2	2.8				1	1
272	1.3 ± 1.9	4.2	4.8b	7.2		4	3
						2.0 ± 1.7	1.7 ± 1.2
VirD2-ssT-DNA
137	17.1 ± 1.5	9.6				1	1
143	40.6 ± 3.5	5.0	10.8			2	2
254	27.5 ± 1.8	2.7	6.0	14.8		3	3
265	22.3 ± 2.1	5.8	8.8			2	2
						2.0 ± 0.8	2.0 ± 0.8

^aThe protein amount is expressed in ng of the GUS protein per 1 g of fresh leaf weight; the data represent mean values (± sd) from three independent experiments.

^bHead-to-tail or tail-to-head integration (4.8 kb).

^cHead-to-head or tail-to-tail integration (4.6 kb).