Figure 3.

LDSP is associated with LDs in Arabidopsis seed and reduces LD size when introduced into the oleo1 mutant. Confocal microscopy of mature wild-type embryos (A and B), oleo1 embryos (C and D), and two independent LDSP-His lines, R5-5 (E and F) and R3-4 (G and H), showing Nile Red fluorescence in the red channel. GFP fluorescence (I), Nile Red fluorescence (J), and merged channels (K) of LDSP-eGFP line Y13 showed the localization of LDSP at the surface of LDs. Bars = 10 μm. Arrows point out bigger LDs with clearly visible ring-shaped GFP fluorescence signal.