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. 2012 Jan 30;158(4):1628–1642. doi: 10.1104/pp.111.191965

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© 2012 American Society of Plant Biologists. All rights reserved.

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Figure 5. — Subcellular localization of GDU1, LOG2, and LOG2 variants. All proteins were transiently expressed in N. benthamiana epidermis cells. Images are maximum projections of optical sections of the abaxial side of cells, obtained by confocal microscopy. A, Localization of GDU1-GFP protein. B, Colocalization of GDU1-GFP and FYVE-RFP, an endosome marker. C, Localization of mLOG2-GFP, mLOG2^G2A-GFP, and mLOG2^R12K-GFP. D, Colocalization of mLOG^G2A with cytosolic mCherry. E, Change of localization of GDU1-GFP when coexpressed with mLOG2-mCherry. The middle cell (arrow) expressed mLOG2-mCherry at a low level, while the two lateral cells expressed mLOG2-mCherry at higher levels. All three cells expressed GDU1-GFP. The central fluorescence of the middle cell is potentially due to internal reflection. Bars = 20 μm (A), 5 μm (B) or 10 μm (C to E).