Figure 7.

Suppression of the Gdu1D phenotype by LOG2-directed amiRNAs in two different Gdu1D overexpression backgrounds. A, Phenotypic segregation on soil of T2 plants expressing LOG2-amiRNA in the gdu1-1D background. Bar = 5 cm. B, Growth of seedlings expressing one of two LOG2-amiRNAs in the gdu1-1D background was assessed for the presence of green, expanded cotyledons after 14 d of growth on 10 mm Met, Leu, and Phe. Error bars represent sd. Asterisks indicate significant differences from gdu1-1D alone (−) on the same medium (t test with Bonferroni correction for multiple comparisons; P < 0.01). Experiments were repeated three or more times with 25 seeds from each line. C, Phenotype of plants overexpressing LOG2-miRNAb in the 35S-GDU1-Myc background. D, For each line overexpressing LOG2-miRNAb in the 35S-GDU1-Myc background, the phenotype of about 10 progeny was recorded (WT, wild type; Inter., intermediate between Gdu1D and the wild type), GDU1 protein levels were estimated by western blotting using an anti-Myc antibody (top), and GDU1 and LOG2 mRNA contents were measured by quantitative RT-PCR. Accumulation is expressed relative to the levels in the wild type (Col-7), and error bars represent the results of two independent experiments. CTRL, Control (35S-GDU1-Myc background only, no amiRNA present). [See online article for color version of this figure.]