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. 2012 Feb 21;158(4):1656–1665. doi: 10.1104/pp.112.193300

Figure 3.

Figure 3.

atHsp93V-ΔN cannot complement the visible phenotypes of an hsp93V mutant. A, Total proteins were extracted from 14-d-old seedlings of the wild type (WT), hsp93V, and two independent lines of hsp93V mutant transformed with atHSP93V or atHSP93V-ΔN grown on MS plates. Thirty micrograms of proteins from each plant was analyzed by immunoblotting using antibodies against Hsp93 and atTic40. The amount of atTic40 was analyzed as a loading control. B and C, Visible phenotypes of plants grown on an MS plate under a 16-h photoperiod for 14 d (B) or grown on soil under a 12-h photoperiod for 26 d (C). D, Chlorophyll contents of seedlings grown on MS plates under a 16-h photoperiod for 15 d. Data shown are means ± sd (n = 3).