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. 2012 Mar 1;158(4):1666–1684. doi: 10.1104/pp.111.192765

Figure 4.

Figure 4.

Functional complementation analysis of the Arabidopsis arr1;arr12 mutant with OsRR22 and OsRR29. A, Schematic of the constructs used in this study. Line, CaMV 35S promoter; light gray boxes, Myc sequence; black boxes, exons; white boxes, introns. B, Root growth inhibition by 1 μm BA. The top panel shows representative 7-d-old seedlings grown in the presence or absence of 1 μm BA. Bar = 1 cm. The bottom panel shows the root lengths of seedlings grown on medium containing 1 μm BA expressed as a percentage of the lengths of siblings grown on dimethyl sulfoxide control medium. Error bars represent se. C, Hypocotyl elongation response to BA of 4-d-old dark-grown seedlings. The hypocotyl length of each line receiving cytokinin treatment is expressed as a percentage of its dimethyl sulfoxide control. Error bars indicate se (n ≥ 8). D, Mean length of seeds from the wild type (Wt), arr1-3;arr12-1, and transgenic lines. Error bars indicate se (n = 80). E, Protein levels of selected response regulator transgenes based on immunoblot analysis using the Myc epitope tag. Hsp70 protein was immunologically detected as a loading control.