Figure 5.

Reproductive abnormalities of mtn1-1mtn2-1. A to F, Scanning electron micrographs of wild-type (A) and manually opened (D) anther sacs showing abnormal pollen development in the mutant. The mtn1-1mtn2-1 pollen grains were irregularly shaped (F) and lacked the characteristic furrow (indicated by the arrow) seen in the wild type (B and C). At times, the mutant pollen appeared clumped with each other (E) compared with the wild type (B). Bars = 45 μm (A and D) and 5 μm (B, C, E, and F). G and H, Differential interference contrast images of stage 14 flower pistils. The wild type had mature ovules in the post fertilization stage (G), while the mtn1-1mtn2-1 ovules (H) corresponded to those normally observed in stage 12 wild-type flowers: outer integuments were not fully extended over the nucellus. Bars = 25 μm.