Figure 3.

Silencing of the AtSQS1 gene and smt2 mutation in Arabidopsis leads to disease susceptibility. A and C, Four-week-old Col-0, smt2 mutant, and SQS1 RNAi (SSi5e) plant leaves were syringe infiltrated with nonhost pathogens Pss or Pta at a concentration of 1 × 10⁷ cfu/mL or dip inoculated with host pathogens Pst DC3000 or Psm at a concentration of 1 × 10⁸ cfu/mL. B and D, Four-week-old plant leaves were syringe inoculated with Pta or Pss at a concentration of 1 × 10⁶ cfu/mL or dip inoculated with Pst DC3000 or Psm at a concentration of approximately 1 × 10⁸ cfu/mL. Bacterial population was quantified by plating serial dilutions of plant leaf extracts at 3 dpi. Asterisks indicate a significant difference from the wild type (Student’s t test; P < 0.01). Data were pooled from two independent experiments representing four biological replicates. Photographs were taken at 4 dpi. See also Supplemental Figure S3.