Table II. Designed primers for four almond ESTs and complete cDNA, annealing temperatures, and expected product size.
Arrows indicate the direction of the primers (→, forward; ←, reverse).
| Accession No. | Sequences (5′–3′) | Temperature | Expected Size |
| °C | bp | ||
| BU572569 | F: TAGCCATGGTTATGCAAAGG (→) | 60 | 525 |
| R: CTGTGGACCAATGGGAACTC (←) | |||
| BU573272 | F: CAACTTTGAATGGGACAATGG (→) | 60 | 600 |
| R: GGGGGCTGAACATTCATTTA (←) | |||
| BU574214 | F: GGCTTTGCTTTGGCAGATAC (←) | 60 | 568 |
| R: GGTCCAATGCTTCACTCGAT (→) | |||
| BU574543 | F: GGCTTTGCTTTGGCAGATAC (→) | 60 | 525 |
| R: CTGCGTATTCTTCAAAGTCATTG (←) | |||
| Ph691cDNA | CAP691F: GGGCACACCTAGCTCGATCATC (→) | 57 | 2,022 |
| 691polyAR: AGAAGAAATTGCATTTATAATTTAGCC (←) | |||
| Ph692cDNA | CAP146972F: GCTGCAAACTTGGACGGTAG (→) | 57 | 1,469 |
| 69LRd5R: GAAGGAGTTGTATTTTATTCATCACAT (←) |