Table I. FA composition of transgenic Arabidopsis seeds of T4 lines expressing castor RcFAH12 and RcROD1.
The transgenic lines of these genes were obtained from independent transformation events. Data represent measurements of three homozygous lines (means ± se).
| Line | FA Composition |
Total HFAa | ||||||||
| 16:0 | 18:0 | 18:1 | 18:2 | 18:3 | 20:1 | 18:1OH | 18:2OH | 20:1OH | ||
| wt % of total | ||||||||||
| Col-0 | 8.0 ± 0.1 | 2.7 ± 0.1 | 15.8 ± 0.2 | 30.6 ± 0.2 | 18.7 ± 0.1 | 21.3 ± 0.1 | ||||
| rod1 | 7.9 ± 0.1 | 2.5 ± 0.1 | 33.5 ± 0.2 | 15.5 ± 0.1 | 13.1 ± 0.1 | 25.3 ± 0.1 | ||||
| rod1-RcROD1 | 7.2 ± 0.5 | 3.0 ± 0.5 | 16.2 ± 1.7 | 29.7 ± 1.2 | 17.6 ± 0.4 | 20.7 ± 0.9 | ||||
| rod1-FAH12 | 12.4 ± 0.6 | 5.2 ± 0.2 | 38.3 ± 0.8 | 14.5 ± 0.3 | 5.9 ± 0.1 | 15.4 ± 0.5 | 2.8 ± 0.1 | 1.2 ± 0.1 | 1.5 ± 0.1 | 5.5 ± 0.1 |
| rod1-FAH12/RcROD1 | 10.1 ± 0.1 | 4.8 ± 0.2 | 25.5 ± 0.3 | 24.3 ± 0.2 | 11.6 ± 0.5 | 11.1 ± 1.5 | 6.2 ± 0.2 | 3.0 ± 0.4 | 0.6 ± 0.1 | 9.7 ± 0.6 |
| Col-FAH12 | 10.1 ± 0.4 | 4.7 ± 0.3 | 19.6 ± 0.4 | 22.2 ± 0.2 | 10.7 ± 0.9 | 17.9 ± 0.2 | 6.3 ± 0.1 | 2.1 ± 0.1 | 1.6 ± 0.1 | 9.9 ± 0.3 |
| Col-FAH12/RcROD1 | 10.0 ± 0.5 | 5.1 ± 0.3 | 24.2 ± 0.7 | 17.5 ± 1.2 | 5.2 ± 0.1 | 14.9 ± 0.6 | 12.7 ± 0.2 | 4.6 ± 0.5 | 2.6 ± 0.1 | 19.9 ± 0.5 |
a
Excluding small amounts of 20:2OH.