Figure 6.

Quantification of small organic acids present in the CaCl₂ extracts of Ler (wt), IG-2, and IG-3 cell cultures before and after elicitation. Ler (wt), IG-2, and IG-3 cell cultures were elicited with purified cell wall extract from F. oxysporum. After elicitation, the cell cultures were filtered and then incubated for 30 min in 200 mm CaCl₂. The supernatant was then recovered, derivatized with ECF or BSTFA, and analyzed by GC-MS, as described in “Materials and Methods.” Data represent averages ± sd of the normalization against Ler (wt).