Figure 7.

Accumulation of H₂O₂, keto acids, and aldehydes following elicitation in Ler (wt) cell cultures. Arabidopsis Ler (wt) cell cultures were resuspended in new medium and pretreated with sodium azide (1 or 2 mm; A) or DPI (2.5 or 25 μm; B) and then elicited with 100 μg mL⁻¹ F. oxysporum extract. Samples were collected every 10 min, and after centrifugation, the supernatants were collected. H₂O₂ was measured using the xylenol orange assay, and keto acids and aldehydes were quantified by DNPH derivatization as described in “Materials and Methods.”