Skip to main content
. Author manuscript; available in PMC: 2012 Apr 5.
Published in final edited form as: Toxicon. 2010 Sep 15;56(4):544–553. doi: 10.1016/j.toxicon.2010.05.012

Table 3.

Factor X activator activity of various snake venoms using factor Xa-specific chromogenic substrate S-2765.

Venom (Avid #) Geographical location Total protein (ng) Specific activity (nkat/ng)a P valueb
B. asper San Antonio Zoo, TX 125 0.051 1.51 × 10−2
B. moojeni Brownsville Zoo, TX 125 0.068 5.84 × 10−3
C. o. helleri 058-359-257 Wrightwood, CA 125 0.059 8.54 × 10−3
D. r. siamensis QSMI, Thailand 125 0.310 3.23 × 10−5
Purified RVV-X 125 1.240 2.41 × 10−5
a

One katal (kat) was the amount of enzyme that converts one mole of chromogenic substrate S-2765 per second. One nanokatal (nkat) was 1 × 10−9 mole of product released per second. Specific activity was calculated by 1 nkat by total protein (ng) in each sample. One nkat was calculated by the following equation: [OD 405 nm/(ε × T × 10−9)], where the enzyme activity is measured at 405 nm, ε is the extinction coefficient of chromogenic substrate S-2765 (1.27 × 104mol−1Lcm−1 at Emax = 405 nm), T is reaction time in second.

b

Their significance was analyzed by the student's t-test. The level of significance was at P < 0.05. P value compared with the negative control.