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GDF-15 expression and secretion by Schwann cells. a–d, Adult Schwann cells isolated from 6-month-old mouse sciatic nerve reveal immunoreactivity for GDF-15. Scale bar, 50 μm. e, Release of GDF-15 from adult human Schwann cells in culture. f, GDF-15 mRNA expression determined by real-time quantitative PCR and normalized against β-actin or S100.
