Table 1.
Size | Forward | Reverse | |
---|---|---|---|
PCR | |||
−/− | 690 bp | 5′-CCC AGT CTT GTA GACAGA GCA A-3′ | 5′-CCC AGT CTT GTA GACAGA GCA A-3′ |
+/+ | 320 bp | 5′-ATG CGC ACC CAAGAG ACT-3′ | 5′-GGC CAC CAG GTCATC ATA AG-3′ |
Probe | 343 bp | 5′-ACT GAT GGG GGAAAG AGG AG-3′ | 5′-TGC CCC ACC CAATATAAA GA-3′ |