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. Author manuscript; available in PMC: 2013 Jan 17.
Published in final edited form as: Biochemistry. 2012 Jan 3;51(2):597–607. doi: 10.1021/bi201221j

Table 2.

Impact of second contact shell mutations on dissociation rate

Mutation koff (mutant) / koff (wild type)
F130L 7,600*
Y54F 73
T106V 11
Q24A 5.0
R84A 3.6
W75F 3.2
H127W 1.6
K121A 1.2
*

12 °C; all others at 37 °C. Biotin dissociation for F130L was too fast to measure above 12 °C.