Changes in Rfrp expression with androgen treatment. A, Representative photomicrographs of Rfrp expression between GDX and GDX with T replaced (GDX + T) and GDX with DHT (GDX + DHT) replaced adult males. 3V, Third ventricle. B, Mean changes in the total number of Rfrp cells and Rfrp mRNA per cell of all cells. C, Mean changes in the HE Rfrp cells and Rfrp mRNA per HE cell. D, Mean changes in the LE Rfrp cells and Rfrp mRNA per LE cell. Bars labeled with different letters designate significantly different groups (P < 0.05). GPC, Grains per cell.