Skip to main content
. 2012 Mar 6;153(4):1638–1648. doi: 10.1210/en.2011-2131

Fig. 1.

Fig. 1.

Characterization of the OSE cell phenotypes of wild-type, Pten;Kras (Trp53+), and Pten;Kras (Trp53-) mice. TRP53 is not expressed in the OSE cell layer of ovaries collected from wild type or Pten;Kras (Trp53−) mice but is elevated in the OSE cells of papillary tumors present invading the ovaries of the Pten;Kras (Trp53+) mice. OSE cells from wild-type mice exhibit a mesothelial-like morphology, are cytokeratin-8 positive, and can proliferate in culture and in Matrigel but are not transformed. In contrast, Pten;Kras (Trp53+) OSE cells grow in elaborate papillary-like, cytokeratin-8-positive structures in vivo and when cultured in Matrigel. The Trp53 depleted OSE cells of the Pten;Kras (Trp53−) mice exhibit limited papillary-like structures in vivo and in Matrigel. A, Immunolabeling of TRP53 of sections from ovaries of WT and mutant mice. Areas of magnification (×40) are indicated by boxes. B, Immunolabeling of the epithelial specific marker cytokeratin-8 of sections from ovaries of wild-type and mutant mice. C, Higher magnification (×20) of the epithelial layer. D, Bright-field image of isolated OSE cells grown in cell culture dishes. E, H&E staining of sections from isolated OSE cells grown in Matrigel for 10 d.