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. 2012 Mar 6;153(4):1638–1648. doi: 10.1210/en.2011-2131

Fig. 4.

Fig. 4.

Pten;Kras;(Trp53+) and Pten;Kras;(Trp53−) mutant OSE cells are transformed, yet only Pten;Kras;(Trp53+) cells are locally invasive. A, Trp53+ and Trp53− OSE cells form similar numbers of colonies in soft agar. B, Pten;Kras;(Trp53+) and Pten;Kras;(Trp53−) mutant OSE cells form sc ectopic tumors, yet only the Trp53+ OSE cells invade the dermal and epidermal layers of the skin as shown by the damaged tissue and the presence of the cytokeratin-8 immunolabeled tumor cells in the dermal and epidermal layers of the skin. The black line demarcates the cytokeratin-8-positive tumors cells and cytokeratin-8-negative dermal and epidermal cells. C, Average volume of six ectopic tumors formed 20 d after sc injection of 1 × 106 Trp53+ or Trp53− OSE cells.