Fig. 3.

Adipogenesis in vitro. MEF were generated and differentiated into adipocytes in vitro. Differentiation was determined with Oil red-O staining and gene expression. A, Oil red-O staining of lipid in differentiated cells. Lipid is indicated by the red color in the cytoplasm. Heterozygous KO MEF (SIRT1^+/−) were used in the control together with WT MEF. B, PPARγ and C/EBPα protein in differentiated cells. The proteins were determined in Western blotting. C, mRNA of PPARγ and adiponectin in differentiated cells. Values are the means ± se (n = 6). *, P ≤ 0.05.