Fig. 2.

Confirmation of sexually differentiated Kiss1 mRNA expression from AVPV/PeN micropunches in adult male (M) and female (F) mice. Animals were GDX and E₂ treated for 1 wk in adulthood. A, Representative ethidium bromide-stained gel showing AVPV/PeN Kiss1 expression is higher in females than males. Gapdh expression is shown as a loading control. B, Mean Kiss1 expression normalized to Gapdh in micropunches taken from the AVPV/PeN of males and females, as determined by qPCR. Females express significantly more Kiss1 mRNA from AVPV/PeN micropunches than males. *, Significantly different from females (P < 0.05).