Fig. 6.

CpG methylation analysis of Kiss1 CpG in the ARC of mice that were GDX and E₂ treated in adulthood before brain collection. A, Mean Kiss1 expression normalized to Gapdh as determined by qPCR of micropunches taken from the ARC of males (M) and females (F). No sex difference in Kiss1 gene expression was detected in the ARC micropunches. B, Mean CpG methylation levels in the ARC Kiss1 gene of four CpG that were found to be sexually dimorphic in the AVPV/PeN. Unlike in the AVPV/PeN, no significant sex differences were detected in the Kiss1 methylation levels of any of the four CpG in the ARC region.